Abstract
ABSTRACTPeach (Prunus persica) is an economically significant fruit-bearing tree. The trichomes of peach fruits are essential for their growth and development by playing a protective role against abiotic stresses, such as intense ultraviolet (UV) light, high temperature, cold-induced stress, and biotic stresses, such as pests and disease infestation. However, the mechanism underlying trichome development in peach fruits is unknown. Therefore, spatial transcriptome sequencing technology was used to compare the transcriptomic information seven days after flowering (DAF) of peach and nectarine fruits at an ultra-high resolution. The results revealed significant variations in how fruits at the early development stage responded to stress exposure. Comparatively, nectarine response to stress was significantly magnified than peach. Notably, a novel trichome-related marker gene,Prupe.7G196500, was identified in peach, which showed a robust function in response to jasmonic acid and wounding. Further, thePrupe.7G196500pro::GUSconstruct was found to specifically expressed in the trichomes of the true leaves of 10-day-oldArabidopsis thaliana(Arabidopsis) seedlings and induced by drought treatment. Finally, gain-of-function analysis showed thatPrupe.7G196500promoted trichomes development. In conclusion, this study identified the cell types and novel marker genes associated with peach fruit. The role ofPrupe.7G196500in positively regulating trichome development was also characterized, thereby laying the foundation for analyzing the mechanism involved in trichome development in peach fruits.
Publisher
Cold Spring Harbor Laboratory