Measuring mtDNA turnover, synthesis, and supercoiling via selective bromodeoxyuridine incorporation

Abstract

AbstractIncreasing work on mitochondria is focused on the dynamic aspects of this organelle, a key part of which is regulation of the mitochondrial genome (mtDNA). Here we describe a protocol that selectively incorporates bromodeoxyuridine into mtDNA for the measurement of mtDNA turnover, synthesis or supercoiling via an adapted immunoblot and Southern blot protocol. For complete use and execution of this protocol see Al Khatib et al.1,2and Lei et al.3