Co-transcriptional production of programmable RNA condensates and synthetic organelles

Abstract

AbstractCondensation of RNA and proteins is central to cellular functions, and the ability to program it would be valuable in synthetic biology and synthetic cell science. Here we introduce a modular platform for engineering synthetic RNA condensates from tailor-made, branched RNA nanostructures that fold and assemble co-transcriptionally. Up to three orthogonal condensates can form simultaneously and selectively accumulate guest molecules. The RNA condensates can be expressed within synthetic cells to produce membrane-less organelles with controlled number, size, morphology and compositions, and that display the ability to selectively capture proteins. Thein situexpression of programmable RNA condensates could underpin spatial organisation of functionalities in both biological and synthetic cells.