CC-seq: Nucleotide-resolution mapping of Spo11 DNA double-strand breaks inS. cerevisiaecells

Abstract

i.SummaryDuring meiosis Spo11 generates DNA double-strand breaks to induce recombination, becoming covalently attached to the 5′ ends on both sides of the break during this process. Such Spo11 “covalent complexes” are transient in wild-type cells, but accumulate in nuclease mutants unable to initiate repair. The CC-seq method presented here details how to map the location of these Spo11 complexes genome-wide with strand-specific nucleotide-resolution accuracy in synchronisedSaccharomyces cerevisiaemeiotic cells.