RNF213 variant and autophagic impairment: A pivotal link to endothelial dysfunction in Moyamoya disease

Abstract

AbstractBackgroundMoyamoya disease (MMD) is closely associated with the Ring Finger Protein 213 (RNF213), a susceptibility gene for this disease. However, its biological function remains unclear. We aimed to elucidate the role of RNF213 in the damage incurred by human endothelial cells under oxygen-glucose deprivation (OGD), a condition that mimics intracranial ischemia in patients with MMD.MethodsWe analyzed autophagy in peripheral blood mononuclear cells (PBMCs) derived from patients carrying either RNF213 wild-type (WT) or variant (R4810K). Subsequently, human umbilical vein endothelial cells (HUVECs) were transfected with RNF213 WT (HUVECWT) or R4810K (HUVECR4810K) and exposed to OGD for 2 h to determine the role of the RNF213 variant in such a setting. Immunoblotting was used to analyze autophagy marker proteins, and tube formation assays were performed to examine endothelial function. Autophagic vesicles were observed using transmission electron microscopy. Post-OGD exposure, we administered autophagy modulators such as rapamycin and cilostazol.ResultsThe RNF213 variant group during post-OGD exposure (vs. pre-OGD exposure) showed autophagy inhibition, increased protein expression of SQSTM1/p62 (p< 0.0001) and LC3-II (p= 0.0039), and impaired endothelial function (p= 0.0252). HUVECR4810Kduring post-OGD exposure (versus pre-OGD exposure) showed a remarkable increase in autophagic vesicles. Administration of autophagy modulators notably restored the function of HUVECR4810Kand cellular autophagy.ConclusionsOur findings support the pivotal role of autophagy impaired by the RNF213 variant in MMD-induced endothelial cell dysfunction and underscore the critical mechanism of autophagy leading to progressive endothelial dysfunction and MMD pathogenesis under relative ischemia within the intracranial portion.