Abstract
ABSTRACTCentenarians provide a unique lens through which to study longevity, healthy aging, and resiliency. Moreover, models ofhumanaging and resilience to disease that allow for the testing of potential interventions are virtually non-existent. We obtained and characterized over 50 centenarian and offspring peripheral blood samples including those connected to functional independence data highlighting resistance to disability and cognitive impairment. Targeted methylation arrays were used in molecular aging clocks to compare and contrast differences between biological and chronological age in these specialized subjects. Isolated peripheral blood mononuclear cells (PBMCs) were then successfully reprogrammed into high-quality induced pluripotent stem cell (iPSC) lines which were functionally characterized for pluripotency, genomic stability, and the ability to undergo directed differentiation. The result of this work is a one-of-a-kind resource for studies of human longevity and resilience that can fuel the discovery and validation of novel therapeutics for aging-related disease.
Publisher
Cold Spring Harbor Laboratory
Reference80 articles.
1. Aging, Natural Death, and the Compression of Morbidity
2. Compression of Morbidity 1980–2011: A Focused Review of Paradigms and Progress;Journal of Aging Research,2011
3. Centenarians: the older you get, the healthier you have been;The Lancet,1999
4. Morbidity Profiles of Centenarians: Survivors, Delayers, and Escapers
5. Centenarians as Models of Resistance and Resilience to Alzheimer’s Disease and Related Dementias;Adv Geriatr Med Res,2020