Proteome analysis provides insights into sex differences inHolothuria Scabra

Author:

Cheng Chuhang,Wu FeiFeiORCID,Xu Yizhi,Ren Chunhua,Chen Ting,Li Shella,Shen PeihongORCID,Jiang Fajun

Abstract

AbstractSex-determining mechanism is still ambiguous for sea cucumberHolothuria scabrawhich only manifests gonochorism in gonad. In this study, proteomic analysis was employed to delineate sex-related proteins and genes in gonads ofH. scabra, subsequently validated through Quantitative real-time polymerase chain reaction (qRT-PCR). A total of 5,313 proteins were identified via proteome sequencing. Among these, 817 proteins exhibited expression in both the ovary and testis, with 445 proteins displaying up-regulation and 372 proteins showing down-regulation. Furthermore, 136 and 69 proteins were identified as ovary-specific and testis-specific Differentially Abundant Proteins (DAPs), respectively. For the validation of 75 DAP coding genes, 9 genes were considered to be reliable. Notably, 25 ovary-bias proteins enriched in ribosome pathway strongly indicated the crucial role of ribosome in ovary. And 5S/18S rRNA ratio inH. Scabramay have potencial to establish a nondestructive method to distinguish sexes unambiguously. This study serves to furnish novel evidence pertaining to sex differences inH. scabra.

Publisher

Cold Spring Harbor Laboratory

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