Measuring the time-dependent effects of dobutamine on the modulations of cardiac activity in rats

Abstract

AbstractThe measurement of the autonomic modulations of cardiac dynamics plays a crucial role in the understanding of cardiovascular health and disease. We aimed to estimate the time-resolved sympathetic and parasympathetic modulations of cardiac dynamics in a rat models. In this study, we present the adaptation of the method for rat cardiac analysis and compare it with standard measures of low-frequency (LF) and high-frequency (HF) components, which are commonly used for sympathetic and parasympathetic activity estimation, respectively.To evaluate the performance of our method, we study a dataset comprising spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). These rats were administered dobutamine to elicit autonomic dynamics. The results obtained from our method demonstrated accurate time-resolved depiction of sympathetic reactivity induced by dobutamine administration. These responses closely resembled the expected autonomic alterations observed during physical exercise conditions, albeit emulated pharmacologically. The comparisons with LF and HF measures further confirmed the effectiveness of our method in better capturing autonomic changes in rat cardiac dynamics. Our findings highlight the potential of our adapted method for time-resolved analysis in future clinical and translational studies involving rodents’ models. The validation of our approach in animal models opens new avenues for investigating the relationship between ongoing changes in cardiac activity and parallel changes in brain dynamics. Such investigations are crucial for advancing our understanding of the intricate brain-heart connection, particularly in cases involving neurodegeneration, brain injuries, and cardiovascular conditions.