Abstract
AbstractAntibiotic-resistantPseudomonas aeruginosais a common nosocomial pathogen all over the world. We detected the presence ofP. aeruginosain 22% (53 out of 238) of the test samples collected from patients with infections including secondary wound infections, abscesses and urinary tract infections admitted to two academic hospitals in Bangladesh. Resistance to carbapenems (imipenem, and meropenem) was present among 30% (16 out of 53) of these clinicalP. aeruginosaisolates, which is more than 2-fold higher compared to that of previous studies. Such a rapid increase in carbapenem resistance was mediated by metallo-β-lactamase (MBL). Expression of MBL was detected in 90% (14 out of 16) of these resistant isolates. Molecular analyses revealed that the carbapenem-resistant isolates carried at least one of the MBL variants, eitherbla-VIM orbla-NDM-1. All thebla-NDM-1 positives carried a 0.5 MDa plasmid. ERIC-PCR revealed the highly heterogeneous nature of theP. aeruginosaisolates indicating multiple sources of infection within the hospital. However, the majority of XDR isolates belonged to a single cluster of drug-resistant bacterial infections. These findings indicate that Metallo-β-lactamase (MBL) mediated resistance to carbapenem inP. aeruginosaposes a serious threat to the spread of infections among hospitalized patients.
Publisher
Cold Spring Harbor Laboratory