DIETARY REGULATION OF SILENT SYNAPSES IN THE DORSOLATERAL STRIATUM

Abstract

ABSTRACTObesity results in circuit adaptations that closely resemble those induced by drugs of abuse. AMPA-lacking ‘silent’ synapses are critical in circuit generation during early development, but largely disappear by adulthood. Drugs of abuse increase silent synapses during adulthood and may facilitate the reorganization of brain circuits around drug-related experience, facilitating addiction and relapse Whether obesity causes addiction-related synaptic circuit reorganization via alterations in silent synapse expression has not been examined. Using a dietary-induced obesity paradigm, we show that mice that chronically consumed high-fat diet (HFD) exhibit upregulated silent synapses in both direct and indirect pathway medium spiny neurons in the dorsolateral striatum. Both the onset of silent synapses and their re-silencing after HFD withdrawal occur on an extended time scale of weeks rather than days. Our data suggest that HFD-related silent synapses likely arise from AMPA receptor internalization rather than throughde novosynaptogenesis of NR2B-containing NMDA receptors. These data demonstrate that chronic consumption of high-fat diet can alter mechanisms of circuit plasticity, likely facilitating neural reorganization analogous to that observed with drugs of abuse.