Exonuclease action of replicative polymerase gamma drives damage-induced mitochondrial DNA clearance

Abstract

AbstractMitochondrial DNA (mtDNA) replication is essential for mitochondrial function. This is carried out by a dedicated DNA polymerase gamma, with 5’-3’ polymerase and 3’-5’ proofreading/ exonuclease activity. Perturbations to either properties can have pathological consequences. Predominant sources for replication stress are DNA lesions, such as those induced by oxidative damage. How mtDNA lesions affect the polymerase activity and mtDNA stabilityin vivois not fully understood. To address this, we induce mtDNA-specific damage inS. cerevisiae.We observe that mtDNA damage results in significant mtDNA loss. This loss occurs independent of cell cycle progression or cell division, suggesting an active mechanism for damaged mtDNA clearance. We implicate the 3’-5’ exonuclease activity of the mtDNA polymerase in this clearance, with rates of loss being affected by cellular dNTP levels. We propose a model where polymerase encounter with DNA lesions transitions its activity from synthesis to degradation, resulting in damaged mtDNA clearance. Overall, our findings reveal context-dependent, selective regulation of two critical but opposing functions of polymerase gamma to ensure mitochondrial genome integrity.