Functional characterization of Lipid storage droplets 1 (LSD1) in growth and lipolysis ofHermetia illucens

Abstract

AbstractAs intracellular organelles in adipose tissue, lipids droplets manage the balance between triglyceride accumulation and energy consumption in animals. Perilipin family members, associated with surface of lipid droplets, participate the regulation of lipid metabolism. Lipid storage droplet-1 (LSD1)/Perilipin-1 acts as a gatekeeper for adipose lipid storage in animals. Despite extensive studies in fruit fly, the function ofLSD1in insect larval stage remain indistinct. In this study, we characterized the function ofLSD1in black soldier flyHermetia illucens, a nova resource insect to recycle organic wastes. We found thatLSD1was broadly present in dipteran species and evolved with divergence between mosquitos and flies. We further constructedin vivomutagenesis mediated by CRISPR/Cas9 and found that mutations inLSD1increased the larval weight and did not bring any defects in development. Raw fat content was also not significantly influenced in late larval stage and new-emerged adults. Our results not only extend our knowledge ofLSD1in insects, but also help for better understanding of the lipid homeostasis in BSF.