JAK-STAT pathway activation compromises nephrocyte function in aDrosophilahigh-fat diet model of chronic kidney disease

Abstract

ABSTRACTChronic kidney disease is a major healthy issue and is gaining prevalence. Using aDrosophilamodel for chronic kidney disease we show that a high-fat diet (HFD) disrupts the slit diaphragm filtration structure in nephrocytes, the fly functional equivalent of mammalian podocytes. The structural disruption resulted in reduced filtration function in the affected nephrocytes. We demonstrate that a HFD activates the JAK-STAT pathway in nephrocytes, which has previously been linked to diabetic kidney disease. JAK-STAT activation was initiated by increased expression and release of the adipokine, Upd2, from the fat body. This leptin-like hormone is a known ligand of JAK-STAT. Both genetic and pharmacological inhibition of JAK-STAT restored nephrocyte HFD-associated dysfunction. Altogether, our study reveals the importance of the JAK-STAT signaling pathway in the adipose tissue−nephrocyte axis and its contribution to HFD-associated nephropathy. These findings open new avenues for intervention in treating diabetic nephropathy and chronic kidney disease.HIGHLIGHTSHigh-fat diet (HFD) disrupt nephrocyte slit diaphragm structure and filtrationHFD releases fat body adipokine, Upd2, which activates JAK-STAT in nephrocytesGenetic/pharmacological inhibition of JAK-STAT reverses HFD nephrocyte dysfunctionJAK-STAT signaling mediates adipose-nephrocyte axis in HFD-associated nephropathyIMPACT STATEMENTUsing aDrosophilamodel for chronic kidney disease, Zhao et al. show that a high-fat diet induces excretion of a leptin-like JAK-STAT ligand from the fat body. Thus, driving the adipose-nephrocyte (podocyte equivalent) axis through activated JAK-STAT signaling. These findings link obesity to kidney disease, implicating new avenues for therapeutics.