Neutralization Assay with SARS-CoV-1 and SARS-CoV-2 Spike Pseudotyped Murine Leukemia Virions-Reference-Cited by-同舟云学术

Neutralization Assay with SARS-CoV-1 and SARS-CoV-2 Spike Pseudotyped Murine Leukemia Virions

Published:2020-07-18 Issue: Volume: Page:
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Author:

Zheng Yue,Larragoite Erin T.,Lama Juan,Cisneros Isabel,Delgado Julio C.,Slev Patricia,Rychert Jenna,Innis Emily A.,Williams Elizabeth S.C.P.,Coiras Mayte,Rondina Matthew T.,Spivak Adam M.,Planelles Vicente^ORCID

Abstract

AbstractAntibody neutralization is an important prognostic factor in many viral diseases. To easily and rapidly measure titers of neutralizing antibodies in serum or plasma, we developed pseudovirion particles composed of the spike glycoprotein of SARS-CoV-2 incorporated onto murine leukemia virus capsids and a modified minimal MLV genome encoding firefly luciferase. These pseudovirions provide a practical means of assessing immune responses under laboratory conditions consistent with biocontainment level 2.

Publisher

Cold Spring Harbor Laboratory

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