Author:
Trinh Bon Q.,Ummarino Simone,Ebralidze Alexander K.,van der Kouwe Emiel,Bassal Mahmoud A.,Nguyen Tuan M.,Coffey Rory,Tenen Danielle E.,Fabiani Emiliano,Gurnari Carmelo,Wu Chan-Shuo,Angarica Vladimir Espinosa,Zhang Yanzhou,Ying Li,Yang Henry,Heller Gerwin,Chen Sisi,Zhang Hong,Thurm Abby R.,Marchi Francisco,Levantini Elena,Staber Philipp B.,Zhang Pu,Voso Maria Teresa,Pandolfi Pier Paolo,Ruscio Annalisa Di,Tenen Daniel G.
Abstract
ABSTRACTThe mechanism underlying cell type-specific gene induction conferred by ubiquitous transcription factors as well as disruptions caused by their chimeric derivatives in leukemia is not well understood. Here we investigate whether RNAs coordinate with transcription factors to drive myeloid gene transcription. In an integrated genome-wide approach surveying for gene loci exhibiting concurrent RNA- and DNA-interactions with the broadly expressed transcription factor RUNX1, we identified the long noncoding RNA LOUP. This myeloid-specific and polyadenylated lncRNA induces myeloid differentiation and inhibits cell growth, acting as a transcriptional inducer of the myeloid master regulator PU.1. Mechanistically, LOUP recruits RUNX1 to both the PU.1 enhancer and the promoter, leading to the formation of an active chromatin loop. In t(8;21) acute myeloid leukemia, wherein RUNX1 is fused to ETO, the resulting oncogenic fusion protein RUNX1-ETO limits chromatin accessibility at the LOUP locus, causing inhibition of LOUP and PU.1 expression. These findings highlight the important role of the interplay between cell type-specific RNAs and transcription factors as well as their oncogenic derivatives in modulating lineage-gene activation and raise the possibility that RNA regulators of transcription factors represent alternative targets for therapeutic development.KEY POINTSlncRNA LOUP coordinates with RUNX1 to induces PU.1 long-range transcription, conferring myeloid differentiation and inhibiting cell growth.RUNX1-ETO limits chromatin accessibility at the LOUP locus, causing inhibition of LOUP and PU.1 expression in t(8;21) AML.
Publisher
Cold Spring Harbor Laboratory
Cited by
1 articles.
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