Modelling the nuclear envelope ofHeLacells

Abstract

AbstractThis paper describes a framework for the automatic segmentation of the nuclear envelope of cancerous HeLa cells and the modelling of the volumetric shape against an ellipsoid. The framework is automatic and unsupervised and reported a Jaccard Similarity Index of 0.968 against a manual segmentation. The modelling of the surface provides a visual display of the variations, both smooth and rugged over the surface, and measurements can be extracted with the expectation that they can correlate with the biological characteristics of the cells.