Author:
Wood George,Powell Jessica,Johnson Rebecca,Lastovka Filip,Bryant Owain,Brember Matt,Tourlomousis Pani,Carr John,Bryant Clare,Chung Betty Y-W
Abstract
AbstractSalmonellais a globally important pathogen that actively invades and replicates within host cells, including epithelial cells and macrophages, during infection. Bacterial internalization is predominantly orchestrated by theSalmonellaSPI-1 Type III secretion system (T3SS). The SPI-1 T3SS transports effectors directly through the host cell membrane and into its cytosol to triggerSalmonellauptake. Here, we demonstrate thatSalmonellarapidly synthesizes SPI-1 components as they encounter potential host macrophages, priming the bacteria for host invasion. Assembly of the T3SS in the host membrane results in transient pore formation. We show that this leads to the surge of translational induction of host transcription factors, includingEgr1. In macrophages, the rapid synthesis of EGR1 results in the suppression of immune response and pro-apoptotic genes. Thus, SPI-1-mediated membrane damage limits the macrophage immune response toSalmonellainfection
Publisher
Cold Spring Harbor Laboratory
Cited by
1 articles.
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