Abstract
AbstractBackgroundIn 2017-2019, we conducted a large-scale, cluster-randomised trial (LLINEUP) to evaluate long-lasting insecticidal nets (LLINs) treated with a pyrethroid insecticide plus the synergist piperonyl butoxide (PBO LLINs), as compared to conventional, pyrethroid-only LLINs across 104 health sub-districts (HSDs) in Uganda. In LLINEUP, and similar trials in Tanzania, PBO LLINs were found to provide greater protection against malaria than conventional LLINs, reducing parasitaemia and vector density. In the LLINEUP trial, cross-sectional entomological surveys were carried out at baseline and then every 6 months for two years. In each survey, ten households per HSD were randomly selected for indoor household entomological collections.ResultsOverall, 5395 female Anopheles mosquitoes were collected from 5046 households. The proportion of mosquitoes infected withPlasmodium falciparumdid not change significantly over time, while infection with non-falciparummalaria decreased inAn. gambiaes.s, but notAn. funestus. The frequency of genetic markers associated with pyrethroid resistance increased significantly over time, but the rate of change was not different between the two LLIN types. The knock-down resistance (kdr) mutationVgsc-995S declined over time asVgsc-995F, the alternative resistance mutation at this codon, increased.Vgsc-995F appears to be spreading into Uganda.ConclusionsDistribution of LLINs in Uganda was associated with reductions in parasite prevalence and vector density, but the proportion of infective mosquitoes remained stable, suggesting that the potential for transmission persisted. The increased frequency of markers of pyrethroid resistance indicates that LLIN distribution favoured the evolution of resistance within local vectors and highlights the potential benefits of resistance management strategies.Trial registration: This study is registered with ISRCTN,ISRCTN17516395. Registered 14 February 2017,http://www.isrctn.com/ISRCTN17516395
Publisher
Cold Spring Harbor Laboratory
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