Longitudinal Variability ofBifidobacteriumSpecies in the Infant Gut is Independent of Maternal Milk HMO Composition

Abstract

The development of the infant gut microbiome is primarily influenced by the infant feeding type, with breast milk serving as the optimal source of nutrition. Breast milk contains human milk oligosaccharides (HMOs) that act as nourishment for the developing gut microbiome, potentially conferring advantages to specific bacterial species. Previous studies have demonstrated the ability of certainBifidobacteriumspecies to utilize individual HMOs, however a longitudinal study examining the evolving microbial community at a high resolution in the context of mothers’ milk HMO composition is lacking. Here, we explored the relationship between the HMO composition in mothers’ milk and the abundance ofBifidobacteriumspecies in the infant gut throughout the course of early life. To enable subspecies taxonomic classification, we developed a high-throughput method for quantifying the abundance ofBifidobacterium longumsubsp.infantis(BL. infantis; the best known HMO-utilizer) from metagenomic sequencing. We applied this method to a longitudinal cohort consisting of 21 mother-infant dyads, from whom we collected matched breast milk and infant stool samples at multiple time points during the first year of life. We observed substantial changes in the infant gut microbiome over the course of several months, while the HMO composition in mothers’ milk remained relatively stable.Bifidobacteriumspecies were a prominent factor contributing to the variation observed among samples; however, no significant associations were found between specific HMOs in mothers’ milk and the abundance ofBifidobacteriumspecies. Finally, the longitudinal nature of our cohort enabled us to characterize the dynamic colonization ofBL. infantisin the infant gut, which surprisingly began late in the breastfeeding period. Applying ourBL. infantisquantification method to additional datasets from various geographical locations, we found similar, late-colonization byBL. infantis, highlighting the importance of quantifyingBL. infantisin the infant gut.