Evaluating cytotoxicity and genotoxicity of oil extracted from visceral fat ofCaiman yacare(Daudin, 1802) in chinese hamster lung fibroblastin vitro

Abstract

ABSTRACTIn previous studies, the oil extracted from the visceral fat ofCaiman yacare(Daudin, 1802) demonstrated a wound-healing effect on the skin of Wistar rats. To enhance knowledge our about the mechanism underlying this effect, we analysed the oil’s toxicological potentialin vitro. Cytotoxicity, genotoxicity, pro-oxidant, and antioxidant activities were evaluated in a V79-4 cell line. The oil was obtained using the Soxhlet method, and the proportions of the fatty acid profile was previously identified 43.74 % saturated and 34.65 % unsaturated fatty acids. Protocol 487 of the Organisation for Economic Co-operation and Development (OECD) was employed for cell line selection and concentrations. Cytotoxicity was determined using the MTT assay and clonogenic survival. Pro-oxidant and antioxidant activities were analysed using flow cytometry. Genotoxicity was evaluated using comet and micronucleus assays. The oil did not demonstrate cytotoxicity up to a concentration of 500 µg/mL. At concentrations of 250 and 500 µg/mL, the oil exerted a protective effect against oxidative stress and showed genotoxic effects only at the highest concentration (2000 µg/mL). Like other oils of interest for human health, the oil extracted from the visceral fat ofC. yacaredemonstrated low toxicological potentialin vitro.SUMMARY STATEMENTThe oil fromCaiman yacarevisceral fat presents low cytotoxicity and genotoxicity, highlighting its potential for therapeutic applications without adverse effects.