Diversity and modularity of tyrosine-accepting tRNA-like structures

Abstract

ABSTRACTCertain positive-sense single-stranded RNA viruses contain elements at their 3’ termini that structurally mimic tRNAs. These tRNA-like structures (TLSs) are classified based on which amino acid is covalently added to the 3’ end by host aminoacyl-tRNA synthetase. Recently, a cryoEM reconstruction of a representative tyrosine-accepting tRNA-like structure (TLSTyr) from brome mosaic virus (BMV) revealed a unique mode of recognition of the viral anticodon-mimicking domain by tyrosyl-tRNA synthetase. Some viruses in thehordeivirusgenus ofVirgaviridaeare also selectively aminoacylated with tyrosine, yet these TLS RNAs have a different architecture in the 5’ domain that comprises the atypical anticodon loop mimic. Herein, we present bioinformatic and biochemical data supporting a distinct secondary structure for the 5′ domain of thehordeivirusTLSTyrcompared to those inBromoviridae. Despite forming a different secondary structure, the 5′ domain is necessary to achieve robustin vitroaminoacylation. Furthermore, a chimeric RNA containing the 5′ domain from the BMV TLSTyrand the 3′ domain from ahordeivirusTLSTyrare aminoacylated, illustrating modularity in these structured RNA elements. We propose that the structurally distinct 5′ domain of thehordeivirusTLSTyrs performs the same role in mimicking the anticodon loop as its counterpart in the BMV TLSTyr. Finally, these structurally and phylogenetically divergent types of TLSTyrprovide insight into the evolutionary connections between all classes of viral tRNA-like structures.