Assessing RNAi feasibility and susceptibility to environmental RNAi inTrichogramma dendrolimi(Hymenoptera: Trichogrammatidae)

Author:

Yan Zhichao,Li Fangyi,Wang Aokai,Wang Chengxing,Wang Haiyan,Yu Zeqi,Wang Kepeng,Wang Yihan,Luo Yuanyuan,Li Yuanxi

Abstract

AbstractTrichogramma, a genus of egg parasitoid wasps, are widely used as biological control agents and serve as model organisms in parasitoid research. Despite their significance, the understanding of RNA interference (RNAi) inTrichogrammaremains very limited. In this study, we investigated RNAi-associated genes by bioinformatic approaches and experimentally assessed the feasibility of RNAi and the susceptibility of environmental RNAi inTrichogramma. We found thatTrichogrammagenomes contain a complete set of genes in the RNAi pathway and exhibit extensive gene expansion of dsRNase, which may influence RNAi efficiency by degrading dsRNA. We demonstrated successful RNAi through pupal microinjection inT. dendrolimiMatsumura, providing a technical approach for future gene functional studies. In addition, we observed no evidence of susceptibility to environmental RNAi in eitherT. dendrolimiadults or larvae, which might be attributed to the extensive expansion of dsRNase. This low environmental RNAi sensitivity inTrichogrammacould suggest a reduced risk of RNAi-based pest management strategies affecting nontargetTrichogrammapopulations. Overall, this study presents a technical approach for conducting gene functional studies inTrichogrammaand provides a foundation for evaluating the nontarget effects of RNAi-based pest control strategies onTrichogramma.

Publisher

Cold Spring Harbor Laboratory

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