Clinical and genomic diversity ofTreponema pallidum subsp. pallidum:A global, multi-center study of early syphilis to inform vaccine research

Abstract

AbstractBackgroundThe continuing increase in syphilis rates worldwide necessitates development of a vaccine with global efficacy. We conducted a multi-center, observational study to exploreTreponema pallidumsubsp.pallidum(TPA) molecular epidemiology essential for vaccine research by analyzing clinical data and specimens from early syphilis patients using whole-genome sequencing (WGS) and publicly available WGS data.MethodsWe enrolled patients with primary (PS), secondary (SS) or early latent (ELS) syphilis from clinics in China, Colombia, Malawi and the United States between November 2019 - May 2022. Inclusion criteria included age ≥18 years, and syphilis confirmation by direct detection methods and/or serological testing.TPAdetection and WGS were conducted on lesion swabs, skin biopsies/scrapings, whole blood, and/or rabbit-passaged isolates. We compared our WGS data to publicly available genomes, and analysedTPApopulations to identify mutations associated with lineage and geography.FindingsWe screened 2,820 patients and enrolled 233 participants - 77 (33%) with PS, 154 (66%) with SS, and two (1%) with ELS. Median age of participants was 28; 66% werecis-gender male, of which 43% reported identifying as “gay”, “bisexual”, or “other sexuality”. Among all participants, 56 (24%) had HIV co-infection. WGS data from 113 participants demonstrated a predominance of SS14-lineage strains with geographic clustering. Phylogenomic analysis confirmed that Nichols-lineage strains are more genetically diverse than SS14-lineage strains and cluster into more distinct subclades. Differences in single nucleotide variants (SNVs) were evident byTPAlineage and geography. Mapping of highly differentiated SNVs to three-dimensional protein models demonstrated population-specific substitutions, some in outer membrane proteins (OMPs) of interest.InterpretationOur study involving participants from four countries substantiates the global diversity ofTPAstrains. Additional analyses to exploreTPAOMP variability within strains will be vital for vaccine development and improved understanding of syphilis pathogenesis on a population level.FundingNational Institutes of Health, Bill and Melinda Gates Foundation