Author:
Nishimiya-Fujisawa Chiemi,Petersen Hendrik,Koubková-Yu Tracy Chih-Ting,Noda Chiyo,Shigenobu Shuji,Bageritz Josephine,Fujisawa Toshitaka,Simakov Oleg,Kobayashi Satoru,Holstein Thomas W.
Abstract
AbstractIn many animals, germ cell segregation occurs during early embryogenesis to protect the genome, but its origin in basal metazoans is controversial. Here, we show in the freshwater polypHydraby clonal analysis and transgenic animals that interstitial stem cells comprise two separate stem cell populations, i.e., germline and multipotent somatic stem cells. We isolated genetically labelled stem cells for a global transcriptome study and discovered a broad set of germline-specific/enriched genes includingPrdm9, Pax5, Dmrt1. In an alternative splicing analysis, we identified many genes with germline-specific isoforms; among them, male-specific isoforms ofDmrt1andSnf5. The somatic interstitial stem cell lineage was characterized by numerous neuronal control genes likeNeurog. But all stem cells inHydraalso share a core of stemness genes that has its roots in unicellular eukaryotes. This suggests an evolutionary scenario in which, at the emergence of animal multicellularity, there was an early split into a stable germline and different somatic stem cell lineages.
Publisher
Cold Spring Harbor Laboratory
Cited by
3 articles.
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