Author:
Tsouris Andreas,Brach Gauthier,Schacherer Joseph,Hou Jing
Abstract
SummaryGene expression variation, an essential step between genomic variation and phenotypic landscape, is collectively controlled by local (cis) and distant (trans) regulatory changes. Nevertheless, how these regulatory elements differentially influence the heritability of expression traits remains unclear. Here, we bridge this gap by analyzing the transcriptomes of a large diallel panel consisting of 323 unique hybrids originated from genetically divergent yeast isolates. We estimated the broad- and narrow-sense heritability across 5,087 transcript abundance traits and showed that non-additive components account for 36% of the phenotypic variance on average. By comparing allelic expression ratios in the hybrid and the corresponding parental pair, we identified regulatory changes in 25% of all cases, with a majority acting intrans. We further showed thattrans-regulation could underlie coordinated expression variation across highly connected genes, resulting in significantly higher non-additive variance and most likely in some of the missing heritability of gene expression traits.HighlightsDiallel panel for dissecting genetic components underlying gene expression variationNon-additive components account for 36% of gene expression trait heritabilityMostcis-regulatory variation is buffered by additionaltrans-acting variantsGenes undertrans-regulation show high non-additive variance and functional coherence
Publisher
Cold Spring Harbor Laboratory
Cited by
1 articles.
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