Codon usage bias study of the Vitamin D receptor (VDR) gene of Multiple Sclerosis and Diabetes-1 patients

Abstract

AbstractObjectiveThe codon bias usage study of the 33 Vitamin D Receptor (VDR) genes of the Multiple sclerosis and Diabetes-1 patients were characterized.MethodsVarious computational tools such as clustal-W, Codon adaptation index calculation (CAI cal), Effective number of codons (ENC), Relative Synonymous Codon Usage, Codon usage frequency, Nucleotide substation rate calculation, Relative codon deoptimization index, Grand average hydropathicity, Sequence Manipulation Suite software’s were used to find out the codon usage pattern of the VDR genes in both group of patients.ResultsThe base compositions, nucleotide substation rates, codon adaptation index, hydrophobic nature of the amino acids of VDR gene of both group of patients were analysed.ConclusionThe analysis of the synonymous codon usage pattern of the genes would helpful in the heterologous expression of the VDR genes leads to codon optimizations in Multiple Sclerosis and Diabetes-1 patients. The codon usage bias analysis of the VDR gene of the Multiple sclerosis and Diabetes-1 patients through computational approach determined the pattern of VDR gene expression and evolution during the acquiring of the disease in the patients.