Abstract
AbstractUsing captured CO2and C1-feedstocks like formate and methanol derived from electrochemical activation of CO2are key solutions for transforming industrial processes towards a circular carbon economy. Engineering formate and CO2-based growth in the biotechnologically relevant yeastSaccharomyces cerevisiaecould boost the emergence of a formate-mediated circular bio-economy. This study adopts a growth-coupled selection scheme for modular implementation of the Reductive Glycine Pathway (RGP) and subsequent Adaptive Laboratory Evolution (ALE) to enable formate and CO2assimilation for biomass formation in yeast. We first constructed a serine biosensor strain and then implemented the serine synthesis module of the RGP into yeast, establishing glycine and serine synthesis from formate and CO2. ALE improved the RGP-dependent growth by 8-fold. 13C-labeling experiments reveal glycine, serine, and pyruvate synthesis via the RGP, demonstrating the complete pathway activity. Further, we reestablished formate and CO2-dependent growth in non-evolved biosensor strains via reverse-engineering a mutation inGDH1identified from ALE. This mutation led to significantly more 13C-formate assimilation than in WT without any selection or overexpression of the RGP. Overall, we demonstrated the activity of the complete RGP, showing evidence for carbon transfer from formate to pyruvate coupled with CO2assimilation.
Publisher
Cold Spring Harbor Laboratory
Reference64 articles.
1. Intergovernmental Panel on Climate Change. Global warming of 1.5°C. Special Report on Global Warming of 1.5°C (2018).
2. Production of first and second generation biofuels: A comprehensive review;Renewable and Sustainable Energy Reviews,2010
3. Ritchie, H. , Roser, M. & Rosado, P . CO₂ and Greenhouse Gas Emissions. Our World Data (2020).
4. NBR
1‐mediated p62‐liquid droplets enhance the Keap1‐Nrf2 system
5. Optimizing the Electrochemical Reduction of CO2to Formate: A State-of-the-Art Analysis;ACS Sustain. Chem. Eng,2020
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献