The killing of human gut commensalE. coliED1a by tetracycline is associated with severe ribosome dysfunction

Abstract

SUMMARYRibosomes translate the genetic code into proteins. Recent technical advances have facilitated in situ structural analyses of ribosome functional states inside eukaryotic cells and the minimal bacterium Mycoplasma. However, such analyses of Gram-negative bacteria are lacking, despite their ribosomes being major antimicrobial drug targets. Here we compare twoE. colistrains, a labE. coliK-12 and human gut isolateE. coliED1a, for which tetracycline exhibits bacteriostatic and bactericidal action, respectively. Thein situribosome structures upon tetracycline treatment show a virtually identical drug binding-site in both strains, yet the distribution of ribosomal complexes clearly differs. While K-12 retains ribosomes in a translation competent state, tRNAs are lost in the vast majority of ED1a ribosomes. A differential response is also reflected in proteome-wide abundance and thermal stability assessment. Our study underlines the need to include molecular analyses and to consider gut bacteria when addressing antibiotic mode of action.HIGHLIGHTS•Ribosome structures of gram-negative bacteria are analyzed in situ•Tetracyline is bactericidal to gut isolate despite identical ribosome structures•When antibiotic is bacteriostatic, ribosomal translation competent states are retained•When antibiotic is bactericidal, cells rapidly accumulate P-tRNAs-deficient ribosomesGRAPHICAL ABSTRACT