Abstract
Energy homeostasis is disrupted with age, which then fuels multiple age-related pathologies. The AMP-activated protein kinase (AMPK) is the primary sensor of cellular energy in eukaryotes. However, the genetic regulation of vertebrate aging by AMPK remains poorly understood. Here, we manipulate energy levels in the turquoise killifish by mutating APRT, a key enzyme in AMP biosynthesis. These manipulations produced a male-specific lifespan extension and restored metabolic plasticity. Exploring the observed sex differences using an integrated omics approach implicated the mitochondria as an important player. Mechanistically, APRT regulated mitochondrial functions and AMPK activity, mimicking energy starvation in heterozygous cells. A fasting-like state was also detected, particularly in heterozygous males, which leads to resistance to high-fat diet. Finally, life-long intermittent fasting eliminated the male-specific longevity benefits mediated by the APRT mutation. These observations identify the AMP/AMPK axis as a sex-specific regulator of vertebrate longevity and metabolic health.
Publisher
Cold Spring Harbor Laboratory
Reference157 articles.
1. Plasma glutathione and glutathione disulfide in the rat: regulation and response to oxidative stress;J. Pharmacol. Exp. Ther,1983
2. Ahuja, G. , Bartsch, D. , Yao, W. , Geissen, S. , Frank, S. , Aguirre, A. , Russ, N. , Messling, J.E. , Dodzian, J. , Lagerborg, K.A. , et al. (2019). Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. EMBO Rep 20.
3. Amorim, J.A. , Coppotelli, G. , Rolo, A.P. , Palmeira, C.M. , Ross, J.M. , and Sinclair, D.A . (2022). Mitochondrial and metabolic dysfunction in ageing and age-related diseases. Nat. Rev. Endocrinol. 1–16.
4. Andrews, S. (2017). FastQC: a quality control tool for high throughput sequence data. 2010.
5. The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans
Cited by
9 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献