A low-cost and easy-to-use cell preservation reagent for 4°C or room temperature sample storage

Abstract

AbstractCurrent biospecimen storage and preservation methods no longer meet the demands of basic research and clinical diagnostics. Biospecimen preservation methodology has not advanced to accommodate cutting edge molecular analysis technologies that target single cells and full-length transcripts. Traditional methods, such as flash freezing and formalin-fixed paraffin-embedding (FFPE), were designed to provide information on cell structure and spatial relationships in whole tissues. These methods, however, do not maintain the integrity of proteins and nucleic acids. In this proof-of-concept study we examine preservation mechanisms utilized in nature for survival during cold seasons or periods of drought. Plants, brine shrimp, and tardigrades rely on components such as disaccharides or intrinsically disordered proteins to maintain cellular structure and biological activity in the settings of these environmental stresses. Our study demonstrates that these reagents aid in mammalian cell preservation for at least one week when a sample is stored in solution at 4°C, or as a dried sample in a low humidity desiccator.