Integrated GWAS and Transcriptomic Analysis Identify New Candidate Genes for Seminal Root Growth Angle in Wheat (Triticum aestivumL.)

Abstract

Increased interest in root system architecture (RSA) and its major importance for nutrient and water uptake have intensified the efforts for a detailed study of the different root types within the homorhizic root system found in most monocotyledons as wheat. Mature homorhizic root system comprises two root types regarding their origin: embryonic and pot embryonic. However, knowledge of the different root type’ ss physiology and RSA plasticity is still limited. In wheat, embryonic roots are the first to develop after seed germination and have an important role in crop establishment. Wheat seedlings develop between 3 and 5 embryonic roots that have the same origin, but they differ in their spatial distribution. The first emerging root of a wheat seedling develops from the base of the embryo and grows vertically down. The rest of the seminal roots emerge from the lateral sides of the embryo and grow with a very specific set-point angle dependent on variety. In this study, we showed that seminal roots with different set-point angle displayed differences in response to gravity and in auxin transport. We hypothesized that the differences in RSA among root types will be directed by differences in their transcriptomic profiles. To that end, we performed an RNA sequencing analysis on both root types. With the aim of gaining a more complete understanding of the seminal root architecture plasticity, we also studied the genetic variability associated with root set-point angle performing a Genome-Wide Association Study in a wheat genetic panel of 200 accessions. Our results combined, uncovered a cluster of genes located in Chromosome 2B that comprises new players in wheat RSA with potential roles in plant response to abiotic stress.