Abstract
AbstractThe Grantham’s camellia (Camellia granthamianaSealy) is a rare and endangered tea species that is endemic to southern China, and was first discovered in Hong Kong in 1955. Despite its high conservation value, genomic resources ofC. granthamianaremain limited. Here, we present a chromosome-scale draft genome of the tetraploidC. granthamiana(2n = 4x = 60) using a combination of PacBio long read sequencing and Omni-C data. The assembled genome size is ∼2.4 Gb with most sequences anchored to 15 pseudochromosomes that resemble a monoploid genome. The genome is of high contiguity, with a scaffold N50 of 139.7 Mb, and high completeness with a 97.8% BUSCO score. Gene model prediction resulted in a total 76,992 protein-coding genes with a BUSCO score of 85.9%. 1.65 Gb of repeat content was annotated, which accounts for 68.48% of the genome. The Grantham’s camellia genome assembly provides a valuable resource for future investigations on its biology, ecology, phylogenomic relationships with otherCamelliaspecies, as well as set up a foundation for further conservation measures.
Publisher
Cold Spring Harbor Laboratory
Reference28 articles.
1. Earl Grey: a fully automated user-friendly transposable element annotation and analysis pipeline
2. Beech E , Barstow M , Rivers M. The red list of Theaceae. Botanic Gardens Conservation International; 2017.
3. Sensitive protein alignments at tree-of-life scale using DIAMOND;Nature methods,2021
4. Chen S , Li W , Li W , Liu Z , Shi X , Zou Y , Liao W , Fan Q. Population genetics of Camellia granthamiana, an endangered plant species with extremely small populations in China. Frontiers in Genetics. 2023;14.
5. Haplotype-resolved de novo assembly using phased assembly graphs with hifiasm;Nature methods,2021