Author:
Yamabe Sota,Yoshitake Kazutoshi,Ninomiya Akihiro,Piel Jörn,Takeyama Haruko,Matsunaga Shigeki,Takada Kentaro
Abstract
AbstractNumerous biologically active natural products have been discovered from marine sponges, particularly fromTheonella swinhoei, which is known to be a prolific source of natural products such as polyketides and peptides. Recent studies have revealed that many of these natural products are biosynthesized byCandidatusEntotheonella phylotypes, which are uncultivated symbionts withinT. swinhoei. Consequently, Entotheonella is considered an untapped biochemical resource. In this study, we conducted metagenomic analyses to assess the diversity of Entotheonella in fourT. swinhoeiindividuals (two each of chemotypes Y and W), after separating filamentous bacteria using density gradient centrifugation. We obtained five Entotheonella genomes from filamentous bacteria-enriched fractions. Notably, one of these genomes is significantly different from previously reported Entotheonella variants. Additionally, we identified closely related Entotheonella members present across different chemotypes ofT. swinhoei. Thus, our metagenomic insights reveal that the diversity of Entotheonella withinTheonellasponges is greater than previously recognized.
Publisher
Cold Spring Harbor Laboratory