High-content phenotypic analysis ofa C. elegansrecombinant inbred population identifies genetic and molecular regulators of lifespan

Author:

Gao Arwen W.ORCID,Alam Gaby ElORCID,Zhu Yunyun,Li Weisha,Katsyuba ElenaORCID,Sulc JonathanORCID,Li Terytty Y.,Li XiaoxuORCID,Overmyer Katherine A.ORCID,Lalou Amelia,Mouchiroud Laurent,Sleiman Maroun BouORCID,Cornaglia Matteo,Morel Jean-DavidORCID,Houtkooper Riekelt H.ORCID,Coon Joshua J.ORCID,Auwerx JohanORCID

Abstract

AbstractLifespan is influenced by complex interactions between genetic and environmental factors. Studying those factors in model organisms of a single genetic background limits their translational value for humans. Here, we mapped lifespan determinants in 85 genetically diverseC. elegansrecombinant intercross advanced inbred lines (RIAILs). We assessed molecular profiles – transcriptome, proteome, and lipidome – and life-history traits, including lifespan, development, growth dynamics, and reproduction. RIAILs exhibited large variations in lifespan, which positively correlated with developmental time. Among the top candidates obtained from multi-omics data integration and QTL mapping, we validated known and novel longevity modulators, includingrict-1,gfm-1andmltn-1. We translated their relevance to humans using UK Biobank data and showed that variants inRICTORandGFM1are associated with an elevated risk of age-related heart disease, dementia, diabetes, kidney, and liver diseases. We organized our dataset as a resource (https://lisp-lms.shinyapps.io/RIAILs/) that allows interactive explorations for new longevity targets.

Publisher

Cold Spring Harbor Laboratory

Reference89 articles.

1. A Powerful New Quantitative Genetics Platform, Combining Caenorhabditis elegans High-Throughput Fitness Assays with a Large Collection of Recombinant Strains

2. Experimental evolution reveals antagonistic pleiotropy in reproductive timing but not life span in Caenorhabditis elegans;J Gerontol A Biol Sci Med Sci,2011

3. Automated high-content phenotyping from the first larval stage till the onset of adulthood of the nematode Caenorhabditis elegans;Lab Chip,2018

4. Maximum longevities of chemically protected and non-protected fishes, reptiles, and amphibians support evolutionary hypotheses of aging

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3