Abstract
AbstractMale infertility depends on both sperm and seminal plasma and is induced by aging. In this study, male infertility was examined with seminal plasma and its synthesis mechanism. The factors ensuringin vivofertilization potential was secreted from seminal vesicle where the factors were synthesized in an androgen-dependent manner. Androgen increased glucose uptake and glycolytic capacity in seminal vesicles, which caused activation of oleic acid synthesis rather than mitochondrial ATP synthesis. ACLY was identified as a key player in this metabolic mechanism for producing oleic acid that was incorporated into the sperm and enhanced fertilization potentialin vivo. In conclusion, an important role of testosterone-induced metabolic pathways in the seminal vesicle was to ensure the synthesis of oleic acid, which is essential for sperm fertilizationin vivo. These results provide new perspectives for the development of biochemical markers of semen to assess male fertility and for artificial insemination techniques.One Sentence SummaryTestosterone induces ACLY expression in seminal vesicle, a key factor of forming seminal plasma to acquirein vivofertilization ability of sperm.
Publisher
Cold Spring Harbor Laboratory
Reference59 articles.
1. World Health Organization., Infertility prevalence estimates: 1990–2021. Global report, 1–98 (2023).
2. Epididymal contribution to male infertility: An overlooked problem;Andrologia,2021
3. Review Article: Structure and Function of the Epididymis Transport of Spermatozoa;Urol Res,1986
4. Fertilizing Ability of Cock Spermatozoa from the Testis Epididymis and Vas Deferens Following lntramagnal Insemination
1
,
2
5. Epididymal contribution to male infertility: An overlooked problem;Andrologia,2021