Author:
Dissel Stephane,Morgan Ellen,Cao Lijuan,Wakefield Zachary Peters,Shetty Shohan,Chan Dorothy,Duong Vincent,Donlea Jeff,Farah Hamza,Loutrianakis Vasilios,Ford Melanie,Streett Lillith,Periandri Erica,Li Zhaoyi,Huang Irene,Abdala Dina,Kalra Arjan,Sousani Lea,Holder Brandon,McAdams Chloe,van Swinderen Bruno,Shaw Paul J.
Abstract
AbstractThe relationship between sleep and memory is an active topic of investigation. In this context, we demonstrate that enhancing sleep restores memory to flies with ablated Mushroom Bodies (MB), a key memory center; this is consistent across several memory assays. Mapping the underlying circuitry reveals circadian modulation of a subset of Dopaminergic neurons (DANs) that modulate aversive learning. Using imaging, we show that MB-ablation disrupts, and sleep restores the time of day these neurons are most responsive. Knocking down the receptor for the clock output signal,Pigment-dispersing factor(Pdfr), in this subset of DANs restores memory to MB-ablated flies. Crucially, MB-ablation does not result in memory impairments in the absence of a functioning clock. Our results reveal neuromodulation’s key role in cognitive restoration, where sleep aids memory in damaged brains, but a functioning clock unexpectedly hinders this process.
Publisher
Cold Spring Harbor Laboratory