Yellow and oxidation-resistant derivatives of a monomeric superfolder GFP

Abstract

ABSTRACTFluorescent proteins (FPs) are essential tools in biology. The utility of FPs depends on their brightness, photostability, efficient folding, monomeric state, and compatibility with different cellular environments. Despite the proliferation of available FPs, derivatives of the originally identifiedAequorea victoriaGFP often show superior behavior as fusion tags. We recently generated msGFP2, an optimized monomeric superfolder variant ofA. victoriaGFP. Here, we describe two derivatives of msGFP2. The monomeric variant msYFP2 is a yellow superfolder FP with high photostability. The monomeric variant moxGFP2 lacks cysteines but retains significant folding stability, so it works well in the lumen of the secretory pathway. These new FPs are useful for common imaging applications.