Molecular insights intoVibrio cholerae’s intra-amoebal host-pathogen interactions

Abstract

Vibrio cholerae, which causes the diarrheal disease cholera, is a species of bacteria commonly found in aquatic habitats. Within such environments, the bacterium must defend itself against predatory protozoan grazers. Amoebae are prominent grazers, withAcanthamoeba castellaniibeing one of the best-studied aquatic amoebae. We previously showed thatV. choleraeresists digestion byA. castellaniiand establishes a replication niche within the host’s osmoregulatory organelle. In this study, we deciphered the molecular mechanisms involved in the maintenance ofV. cholerae’s intra-amoebal replication niche and its ultimate escape from the succumbed host. We demonstrated that minor virulence features important for disease in mammals, such as extracellular enzymes and flagellum-based motility, play a key role role in the replication and transmission ofV. choleraein its aqueous environment. This work, therefore, describes new mechanisms that provide the pathogen with a fitness advantage in its primary habitat, which may have contributed to the emergence of these minor virulence factors in the speciesV. cholerae.