Inventory of cultivatable populations of S-cycling, fermentative, Fe-reducing, and aerobic heterotrophic bacteria from salt marsh sediments

Abstract

ABSTRACTA survey was carried out of the dominant chemotrophic groups of bacteria inhabiting surface salt marsh sediments in the Virginia Coastal Reserve (VCR) on the Atlantic coast of Virginia. Total direct cell counts were carried out on all samples. Aerobic heterotrophs, sulfur oxidizers, sulfate reducing bacteria (SRB), sulfur disproportionaters, Fe-reducing (FeRB) and fermentative bacteria were all quantified by most probable number (MPN) at four different sites that ranged in spatial scale from a few meters to 15 km apart. The sites were sampled every 3 – 4 months over a two year period. Total cell counts were quite consistent temporally at each of the sites, and ranged from a high of 1.4 × 1010cells. gdw-1to a low of 8 × 108cells. gdw-1. Recoveries of all culturable bacteria were also site dependent and ranged from a minimum of 0.4% to a maximum of 40% of the total cell count. Aerobic bacteria were the dominant recovered population at all of the sites, followed by sulfur-oxidizing bacteria. Together these two groups accounted for >75% of the total recovered bacteria at each of the sites. The populations of anaerobic groups fluctuated significantly; S-disproportionating and SRB were most abundant followed by FeRB and fermenters. On average, all the anaerobes were in the same order of magnitude of abundance (107cells. gdw-1). Overall, these results suggest that aerobic bacteria consistently predominated in the top 10 cm of the marsh sediments, and that autotrophy related to sulfur oxidation and disproportionation may be important, but under studied processes in salt marsh ecosystems.