Author:
Papin Christophe,Ibrahim Abdulkhaleg,Gras Stéphanie Le,Velt Amandine,Stoll Isabelle,Jost Bernard,Menoni Hervé,Bronner Christian,Dimitrov Stefan,Hamiche Ali
Abstract
DNA methylation is an essential epigenetic modification, present in both unique DNA sequences and repetitive elements, but its exact function in repetitive elements remains obscure. Here, we describe the genome-wide comparative analysis of the 5mC, 5hmC, 5fC, and 5caC profiles of repetitive elements in mouse embryonic fibroblasts and mouse embryonic stem cells. We provide evidence for distinct and highly specific DNA methylation/oxidation patterns of the repetitive elements in both cell types, which mainly affect CA repeats and evolutionarily conserved mouse-specific transposable elements including IAP-LTRs, SINEs B1m/B2m, and L1Md-LINEs. DNA methylation controls the expression of these retroelements, which are clustered at specific locations in the mouse genome. We show that TDG is implicated in the regulation of their unique DNA methylation/oxidation signatures and their dynamics. Our data suggest the existence of a novel epigenetic code for the most recently acquired evolutionarily conserved repeats that could play a major role in cell differentiation.
Funder
CNRS
INSERM
Université de Strasbourg
Université de Grenoble Alpes
ITMO Cancer AVIESAN
INCA
ANR
Fondation pour la Recherche Médicale
Publisher
Cold Spring Harbor Laboratory
Subject
Genetics (clinical),Genetics
Cited by
34 articles.
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