Statistical analysis of number of genes and chromosome lengths of different microbial species

Abstract

AbstractGenome architecture concerns the organisation of genes on a chromosome, and has important implications to the fidelity in which genes are encoded on the chromosome, and how the information is read by DNA polymerase and RNA polymerase. This facet of genomics did receive attention in the early epoch of genomics, but it has received less attention in contemporary genomics as attention shifts to structural and functional genomics with the goal of annotating the function of each gene in the genome. This work sought to uncover relationships between number of genes and chromosome length in a variety of bacteria and archaea species as a preamble to understanding the prevalence and importance of repetitive sequences in the genome of prokaryotic species. Aggregate results with the ensemble of prokaryotic species profiled revealed a positive linear correlation between number of genes and chromosome length. Upon dissection into the Bacteria and Archaea domains, the linear relationship described above still stands for Bacteria but starts to break down in Archaea. This suggests that repetitive sequences are more important to Archaea species, which generally have a smaller genome (1.8 to 2.8 Mbp) and fewer genes (1500 to 2400) compared to bacterial species. In comparison, the bacterial genome is larger (4 to 5.6 Mbp), and encodes more genes (3600 to 5100). Overall, the results highlight that bacterial genome are efficiently encoded with few repetitive sequences. This, however, is not true for archaeal genome, which provides another line of evidence supporting the notion that archaea are ancestral eukaryotic cells, which like the archaea also houses large repetitive sequences.Graphical abstractShort descriptionStatistical analysis across an ensemble of 59 microbial species revealed a strong linear correlation between number of genes and chromosome length. This suggests that prokaryotic genomes are highly compact with genes, and do not carry significant amounts of repeats unlike the case in eukaryotic organisms. The result holds significant implications for our understanding of genome evolution and compaction in prokaryotic organisms, and what drove their accession as foundational species of many ecosystems.Subject areasgenomics, molecular biology, evolutionary biology, bioinformatics, systems biology,