Shadow imaging for panoptical visualization of living brain tissue

Author:

Dembitskaya Yulia,Boyce Andrew K. J.,Idziak Agata,Langeroudi Atefeh Pourkhalili,Bourdellès Guillaume Le,Girard Jordan,Arizono Misa,Ducros Mathieu,Sato-Fitoussi Marie,de Amezaga Amaia Ochoa,Oizel Kristell,Bancelin Stephane,Mercier Luc,Pfeiffer Thomas,Thompson Roger J.,Kim Sun Kwang,Bikfalvi Andreas,Nägerl U. ValentinORCID

Abstract

AbstractProgress in neuroscience research hinges on technical advances in visualizing living brain tissue with high fidelity and facility. Current neuroanatomical imaging approaches either require tissue fixation, do not have cellular resolution or only give a fragmented view. Here, we show how regular light microscopy together with fluorescence labeling of the interstitial fluid in the extracellular space provide comprehensive optical access in real-time to the anatomical complexity and dynamics of living brain tissue.

Publisher

Cold Spring Harbor Laboratory

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