Author:
François Ruthly,Kashamuka Melchior Mwandagalirwa,Banek Kristin,Bala Joseph A.,Nkalani Marthe,Kihuma Georges,Atibu Joseph,Mahilu Georges E.,Thwai Kyaw L.,Assefa Ashenafi,Bailey Jeffrey A.,Dinglasan Rhoel R.,Juliano Jonathan J.,Tshefu Antoinette,Parr Jonathan B.
Abstract
AbstractHistidine-rich protein 2- (HRP2-) based rapid diagnostic tests (RDTs) are widely used to detectPlasmodium falciparumin sub-Saharan Africa. Reports of parasites withpfhrp2and/orpfhrp3 (pfhrp2/3)gene deletions in Africa raise concerns about the long-term viability of HRP2-based RDTs. We evaluated changes inpfhrp2/3deletion prevalence over time using a 2018-2021 longitudinal study of 1,635 enrolled individuals in Kinshasa Province, Democratic Republic of the Congo (DRC). Samples collected during biannual household visits with ≥ 100 parasites/μL by quantitative real-time PCR were genotyped using a multiplex real-time PCR assay. Among 2,726 P.falciparumPCR-positive samples collected from 993 participants during the study period, 1,267 (46.5%) were genotyped. Nopfhrp2/3deletions or mixedpfhrp2/3-intact and -deleted infections were identified in our study.Pfhrp2/3-deleted parasites were not detected in Kinshasa Province; ongoing use of HRP2-based RDTs is appropriate.
Publisher
Cold Spring Harbor Laboratory
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