Real-time biochemical-free targeted sequencing of RNA species with RISER

Abstract

AbstractWe present the first biochemical-free technology for Real-time In Silico Enrichment of RNA species (RISER). RISER classifies RNA species from direct RNA nanopore signals, without the need for basecalling or a reference, and communicates with the sequencing hardware in real-time to enact in silico targeted RNA sequencing. We illustrate RISER for the enrichment and depletion of coding and non-coding RNA, demonstrating a 3.4-3.6x enrichment and 6.2-6.7x depletion of non-coding RNA in live sequencing experiments.