Developing a temperature-inducible transcriptional rheostat inNeurospora crassa

Abstract

AbstractHeat shock protein (hsp) encoding genes, part of the highly conserved Heat Shock Response (HSR), are known to be induced by thermal stress in several organisms. InNeurospora crassa, threehspgenes,hsp30, hsp70, andhsp80, have been characterized; however, the role of definedcis-elements in their response to discrete changes in temperature remains largely unexplored. To fill this gap, while also aiming to obtain a reliable fungal heat-shock inducible system, we analyzed different sections of eachhsppromoter, by assessing the expression of real-time transcriptional reporters. Whereas all three promoters, and their resected versions, were acutely induced by high temperatures, onlyhsp30displayed a broad range of expression and high tunability amply exciding other inducible promoter systems existing in Neurospora, such as Quinic acid- or light-inducible ones. As proof of concept, we employed one of these promoters to control the expression ofclr-2, which encodes for the master regulator of Neurospora cellulolytic capabilities. The resulting strain fails to grow on cellulose at 25°C, whereas it robustly grows if heat shock pulses are delivered daily. Additionally, we designed twohsp30synthetic promoters and characterized these, as well as the native promoters, to a gradient of high temperatures, yielding a wide range of responses to thermal stimuli. Thus, Neurosporahsp30-based promoters represent a new set of modular elements that can be used as a transcriptional rheostat to adjust the expression of a gene of interest or for the implementation of regulated circuitries for synthetic biology and biotechnological strategies.ImportanceTimely and dynamic response to strong temperature rises is paramount for organismal biology. At the same time, inducible promoters are a powerful tool for fungal biotechnological and synthetic biology endeavors. In this work, we analyzed the activity of severalN. crassaheat shock protein (hsp) promoters upon a wide range of temperatures, observing thathsp30exhibits remarkable sensitivity and dynamic range of expression as we chartered the response of this promoter to subtle increases in temperature, while also building synthetic promoters based onhsp30 cis-elements. As proof of concept, we analyzed the ability ofhsp30to provide tight control of a central process such as cellulose degradation. While this study provides an unprecedented description of the regulation of theN. crassa hspgenes it also contributes with a noteworthy addition to the molecular toolset of transcriptional controllers in filamentous fungi.