Abstract
AbstractBackgroundExtended-spectrum beta-lactamase-producingEscherichia coli(ESBL-PE) represents a significant global public health concern. Much as humans, animals and environments harbor ESBL-PE, its epidemiology in Uganda is still not well understood. This study explains the epidemiology of ESBL-PE using the one health approach in selected farming households in Wakiso district, central Uganda.MethodologyEnvironmental, human, and animal samples were collected from 104 households. Additional data were obtained using observation checklists and through interviews with household members using a semi-structured questionnaire. Surface swabs, soil, water, human and animal fecal samples were introduced onto ESBL chromogenic agar. The isolates were identified using biochemical tests and double-disk synergy tests. To assess associations, prevalence ratios (PRs) were computed using a generalized linear model (GLM) analysis with modified Poisson and a log link with robust standard errors in R software.ResultsA total of 82.7% (86/104) households had at least one positive ESBL-PE isolate. The overall prevalence of ESBL-PE at the human-animal-environment interface was approximately 25.0% (95% CI: 22.7-28.3). Specifically, animals, environment and humans had an ESBL-PE prevalence of 35.4%, 5.8%, and 45.4% respectively. Having visitors (adj PR= 1.19, 95% CI: 1.04-1.36), utilizing veterinary services (adj PR= 1.39, 95% CI: 1.20-1.61) and using animal waste for gardening (adj PR= 1.29, 95% CI: 1.05-1.60) were positively associated with ESBL-PE contamination. However, covering the drinking water container with a lid (adj PR= 0.84 95% CI: 0.73-0.96) was associated with absence of ESBL-PE.ConclusionThere is wider dissemination of ESBL-PE in the environment, humans, and animals, indicating poor infection prevention and control (IPC) measures in the area. Improved collaborative one health mitigation strategies such as safe water chain, farm biosecurity, household and facility-based IPC measures are recommended to reduce the burden of antimicrobial resistance at community level.
Publisher
Cold Spring Harbor Laboratory
Reference30 articles.
1. UBOS, “2014 NPHC-Main Report Main Report National Population and Housing Census 2014,” Uganda Bureau of Statistics, Kampala, Uganda, 2017.
2. UBOS, “The Uganda national household survey,” Uganda Bureau of Statistics, p. 76, 2020.
3. UBoS, “The Republic of Uganda National Livestock Census Report,” Statistics (Ber), p. 256, 2009.
4. A simplified general method for cluster-sample surveys of health in developing countries;World health statistics quarterly 1991,1991
5. A simple method of sample size calculation for linear and logistic regression
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