Free volume theory explains the unusual behavior of viscosity in a non-confluent tissue during morphogenesis

Abstract

A recent experiment on zebrafish blastoderm morphogenesis showed that the viscosity (η) of a non-confluent embryonic tissue grows sharply until a critical cell density (ϕS). The increase inηup toϕSis similar to the behavior observed in several glass forming materials, which suggests that the cell dynamics is sluggish or glass-like. Surprisingly,ηis a constant aboveϕS. To determine the mechanism of this unusual dependence ofηonϕ, we performed extensive simulations using an agent-based model of a dense non-confluent two-dimensional tissue. We show that polydispersity in the cell size, and the propensity of the cells to deform, results in the saturation of the available free area per cell beyond a critical density. Saturation in the free space not only explains the viscosity plateau aboveϕSbut also provides a relationship between equilibrium geometrical packing to the dramatic increase in the relaxation dynamics.