Author:
Trovato Francesco,Parra Riccardo,Pracucci Enrico,Landi Silvia,Cozzolino Olga,Nardi Gabriele,Cruciani Federica,Mosti Laura,Cwetsch Andrzej,Cancedda Laura,Gritti Laura,Sala Carlo,Verpelli Chiara,Maset Andrea,Lodovichi Claudia,Ratto Gian Michele
Abstract
AbstractCre-Lox manipulation is the gold standard for cell-specific expression or knockout of selected genes. However, it is not unusual to deal with conditions of low Cre expression or transient activation, which can often go undetected by conventional Cre-reporters. We designed Beatrix, a general-purpose tool specifically devised to amplify weak Cre recombinase activity, and we used it to develop a powerful approach for thein vivogeneration and detection of sparse mosaics of mutant and wild type cells.
Publisher
Cold Spring Harbor Laboratory
Cited by
1 articles.
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