Anti-oxidant, anti-apoptotic, anti-hypoxic and anti-inflammatory conditions induced by PTY-2 against STZ-induced stress in islets

Abstract

AbstractBackground and AimEarlier assessment of Pueraria tuberosa tubers has shown anti-diabetic effects through incretin mimetic action and DPP-IV inhibition. The aim of this work was to further explore the protective role of aqueous extract of Pueraria tuberosa against streptozotocin (STZ)-induced pancreatic stress in rats.MethodsDiabetes was induced with STZ (65 mg/kg body weight) in Charles foster male rats. After 60 days of STZ administration, animals with blood glucose levels > 200 g/dL were considered as diabetic. All the rats were later divided into three groups: Group-1 (STZ untreated normal rats), Group-2 (Diabetic control), and Group-3 (PTY-2 [50 mg/100 g bw treatment for next 10 days to diabetic rats). The rats were then sacrificed at the 10th day of treatment accordingly.ResultsSTZ treatment led to an increase in expression of MMP-9, Tnf α, HIF-1α, VEGF, IL-6, PKC ε, NF-kB, and Caspase-3. Reverse transcriptase Polymerase Chain Reaction (PCR), IHC and western blot analysis showed an increase in the expressions of superoxide dismutase (SOD) and Nephrin, and a decrease in the expressions of NF-kB, PKC ε, TNF-α MMP-9, HIF-1α, VEGF, Caspase 3 and IL-6 after 10 days of PTY-2 treatment.ConclusionThe results show that PTY-2 favorably changed the expression of NF-kB, PKC ε, TNF α, MMP 9, HIF-1α, VEGF, IL-6, Caspase3, Nephrin and SOD in cases of STZ-induced pancreatic stress. Further evaluation of PTY-2 might be helpful in establishing its role in the management of diabetes mellitus.HighlightsPTY 2 act as a protective herbal drug against STZ induced islet stress.PTY 2 upregulates protective and downregulates harmful markers.This study composed of four pathway through which PTY 2 acts on pancreas.GRAPHICAL ABSTRACTMechanism of action of PTY 2 against STZ induced islet stress.