Single cell RNAseq provides a molecular and cellular cartography of changes to the human endometrium through the menstrual cycle

Author:

Wang WanxinORCID,Vilella FelipeORCID,Alama Pilar,Moreno Inmaculada,Mignardi Marco,Pan WenyingORCID,Simon Carlos,Quake Stephen R.ORCID

Abstract

SummaryIn a human menstrual cycle, the endometrium undergoes remodeling, shedding, and regeneration, all of which are driven by substantial gene expression changes in the underlying cellular hierarchy. Despite its importance in human fertility and regenerative biology, mechanistic understanding of this unique type of tissue homeostasis remains rudimentary. We characterized the transcriptomic transformation of human endometrium at single cell resolution, dissecting the multidimensional cellular heterogeneity of this tissue across the entire natural menstrual cycle. We profiled the behavior of 6 endometrial cell types, including a previously uncharacterized ciliated epithelial cell type, during four major phases of endometrial transformation, and found characteristic signatures for each cell type and phase. We discovered that human window of implantation opens with an abrupt and discontinuous transcriptomic activation in the epithelia, accompanied with widespread decidualized feature in the stromal fibroblasts. These data reveal signatures in the luminal and glandular epithelia during epithelial gland reconstruction, and suggest a mechanism for adult gland formation.

Publisher

Cold Spring Harbor Laboratory

Cited by 6 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Menstruation: science and society;American Journal of Obstetrics and Gynecology;2020-11

2. The Endometrial Transcription Landscape of MRKH Syndrome;Frontiers in Cell and Developmental Biology;2020-09-24

3. Appointment of Carlos Simón as Editor of Preconceptional Care and Biology for AJOG;American Journal of Obstetrics and Gynecology;2020-05

4. The endometrial transcription landscape of MRKH syndrome;2020-02-19

5. Molecular Biology Techniques for Endometrial Gene Expression: Recent Technological Advances;Endometrial Gene Expression;2019-11-09

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